MRPS27 Monoclonal antibody

MRPS27 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1B1B3

Cat no : 66724-1-Ig

Synonyms

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

---

Validation Data Gallery

• 

  WB analysis of LNCaP using 66724-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66724-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66724-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66724-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66724-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66724-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, A431 cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66724-1-Ig targets MRPS27 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRPS27 fusion protein Ag11047
• Full Name: mitochondrial ribosomal protein S27
• Calculated Molecular Weight: 168 aa, 19 kDa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC030521
• Gene Symbol: MRPS27
• Gene ID (NCBI): 23107
• RRID: AB_2882075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Publications

Species	Application	Title
human		Aging (Albany NY); Qualitative and quantitative alterations in intracellular and membrane glycoproteins maintain the balance between cellular senescence and human aging.; Authors - Yoko Itakura
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim
human	WB	Biochim Biophys Acta Mol Basis Dis; LARP1, an RNA-binding protein, participates in ovarian cancer cell survival by regulating mitochondrial oxidative phosphorylation in response to the influence of the PI3K/mTOR pathway; Authors - Jiaoyan Ma