|Positive WB detected in||LNCaP cells, HeLa cells, A431 cells, HepG2 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66724-1-Ig targets MRPS27 in WB, IHC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||MRPS27 fusion protein Ag11047|
|Full Name||mitochondrial ribosomal protein S27|
|Calculated molecular weight||168 aa, 19 kDa, 48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC030521|
|Gene ID (NCBI)||23107|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
Aging (Albany NY)
Qualitative and quantitative alterations in intracellular and membrane glycoproteins maintain the balance between cellular senescence and human aging.
Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema