MRPS31 Polyclonal antibody

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MRPS31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16288-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Imogen 38, IMOGN38, MRP S31, MRP-S31, S31mt

• 

  WB analysis using 16288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16288-1-AP

  WB result of MRPS31 antibody (16288-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS31 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 16288-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16288-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS31 antibody (16288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue, human kidney tissue, human thyroid cancer tissue, mouse lung tissue, mouse kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16288-1-AP targets MRPS31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9434

  Product name: Recombinant human MRPS31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 63-395 aa of BC022045

  Sequence: NSVICSKKDKQSVRTEEISKETSESQDSEKENTKKDLLGIIKGMKVELSTVNVRTTKPPKRRPLKSLEAALGRLRRATEYAPKKRIEPLSPELVAAASAVADSLPFDKQTTKSELLSQLQQHEEESRAQRDAKRPKISFSNIISDMKVARSATARVRSRPELRIQFDEGYDNYPGQEKTDDLKKRKNIFTGKRLNIFDMMAVTKEAPETDTSPSLWDVEFAKQLATVNEQPLQNGFEELIQWTKEGKLWEFPINNEAGFDDDGSEFHEHIFLEKHLESFPKQGPIRHFMELVTCGLSKNPYLSVKQKVEHIEWFRNYFNEKKDILKESNIQFN

• Full Name: mitochondrial ribosomal protein S31
• Calculated Molecular Weight: 395 aa, 45 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC022045
• Gene Symbol: MRPS31
• Gene ID (NCBI): 10240
• RRID: AB_2297732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92665
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MRPS31 Polyclonal antibody (Cat# 16288-1-AP) has 3 citations. These appear in Nature Structural & Molecular Biology, Cell Regeneration, and Human Molecular Genetics. The research fields span mitochondrial ribosome assembly, intestinal epithelial homeostasis and stem cell maintenance, and mitoribosomal protein mutations associated with developmental disorders. All citations used the antibody for Western blot applications in human or mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia
mouse	WB	Cell Regen; eIF5A maintains intestinal epithelial homeostasis by sustaining intestinal stem cells; Authors - Leilei Li
human	WB	Hum Mol Genet; Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.; Authors - Juliette Pulman