MRRF Polyclonal antibody

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• MRRF Polyclonal antibody (12357-2-AP)

MRRF Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12357-2-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

MRFF, mtRRF, Ribosome-recycling factor, mitochondrial, Ribosome-releasing factor, mitochondrial, RRF

• 

  WB analysis using 12357-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12357-2-AP (MRRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 12357-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12357-2-AP (MRRF antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12357-2-AP targets MRRF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3021

  Product name: Recombinant human MRRF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-262 aa of BC013049

  Sequence: MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

• Full Name: mitochondrial ribosome recycling factor
• Calculated Molecular Weight: 262 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC013049
• Gene Symbol: MRRF
• Gene ID (NCBI): 92399
• RRID: AB_2144259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96E11
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MRRF polyclonal antibody (Cat# 12357-2-AP) has 8 citations spanning journals including Nature Communications, EMBO Journal, Journal of Hazardous Materials, Science of the Total Environment, Ecotoxicology and Environmental Safety, Biochemical and Biophysical Research Communications, and Physiological Reports. Research fields cover mitochondrial translation, neurotoxicity, stress granule phase separation, liver cancer metabolism, skeletal muscle biology, and exercise physiology. Applications cited include WB and IF in human, mouse, and rat samples.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.; Authors - Aline Lieber
		Nat Commun; Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts.; Authors - Taisei Wakigawa
rat	WB	J Hazard Mater; ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis; Authors - Zhiyuan Tian
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
		EMBO J; Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism; Authors - Ying Shu
rat	WB	Ecotoxicol Environ Saf; Mitochondrial translation impairment-triggered neuroinflammation mediates fluoride-induced cognitive deficits.; Authors - Wenhui Liu