MRRF Monoclonal antibody, PBS Only

• 

  WB analysis using 66942-1-Ig (same clone as 66942-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66942-1-Ig (MRRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66942-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66942-1-Ig (same clone as 66942-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRRF antibody (66942-1-Ig, Clone: 1G5C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66942-1-PBS in a different storage buffer formulation.

MRRF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66942-1-PBS

Clone No.1G5C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RRF, Ribosome-releasing factor, mitochondrial, Ribosome-recycling factor, mitochondrial, mtRRF, MRFF

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Product Information

66942-1-PBS targets MRRF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28583

  Product name: Recombinant human MRRF protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-262 aa of BC013049

  Sequence: MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

• Full Name: mitochondrial ribosome recycling factor
• Calculated Molecular Weight: 262 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC013049
• Gene Symbol: MRRF
• Gene ID (NCBI): 92399
• RRID: AB_2882266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96E11
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.