Validation Data Gallery
|Positive WB detected in
|Daudi cells, Raji cells, Ramos cells
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue, Raji cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
24828-1-AP targets CD20 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|CD20 fusion protein Ag17309
|membrane-spanning 4-domains, subfamily A, member 1
|Calculated molecular weight
|297 aa, 33 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
|Product Specific Protocols
|WB protocol for CD20 antibody 24828-1-AP
|IHC protocol for CD20 antibody 24828-1-AP
|IF protocol for CD20 antibody 24828-1-AP
|IP protocol for CD20 antibody 24828-1-AP
|Click here to view our Standard Protocols
J Immunother Cancer
Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.
Clin Exp Immunol
CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.
Acta Physiol (Oxf)
Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.
Int J Mol Sci
CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.