CD20 Polyclonal antibody
CD20 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IHC, IF, FC, ELISA
Cat no : 24828-1-AP
Validation Data Gallery
|Positive WB detected in||Daudi cells, Raji cells, Ramos cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, Raji cells|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
24828-1-AP targets CD20 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD20 fusion protein Ag17309|
|Full Name||membrane-spanning 4-domains, subfamily A, member 1|
|Calculated molecular weight||297 aa, 33 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002807|
|Gene ID (NCBI)||931|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
|Product Specific Protocols|
|WB protocol for CD20 antibody 24828-1-AP||Download protocol|
|IHC protocol for CD20 antibody 24828-1-AP||Download protocol|
|IF protocol for CD20 antibody 24828-1-AP||Download protocol|
|IP protocol for CD20 antibody 24828-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Immunother Cancer
Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.
Clin Exp Immunol
CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.
Acta Physiol (Oxf)
Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.