CD20 Polyclonal antibody

CD20 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 24828-1-AP

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A2, S7



Tested Applications

Positive WB detected inRaji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24828-1-AP is CD20 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002807
Gene symbol MS4A1
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD20 antibody 24828-1-APDownload protocol
IHC protocol for CD20 antibody 24828-1-APDownload protocol
IF protocol for CD20 antibody 24828-1-APDownload protocol
IP protocol for CD20 antibody 24828-1-APDownload protocol
FC protocol for CD20 antibody 24828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Physiol (Oxf)

Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.

Authors - A Zietzer