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MSH2 Monoclonal antibody

MSH2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2H5F5

Cat no : 60161-1-Ig

Synonyms

COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2



Tested Applications

Positive WB detected inColo320 cells, HeLa cells, A2780 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60161-1-Ig targets MSH2 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC021566
Gene symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
WB protocol for MSH2 antibody 60161-1-IgDownload protocol
IHC protocol for MSH2 antibody 60161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols