MSL2 Polyclonal antibody

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• MSL2 Polyclonal antibody (23601-1-AP)

• 

  WB analysis using 23601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23601-1-AP (MSL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 23601-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSL2 antibody (23601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MSL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23601-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MS L2, MSL 2, MSL2 like 1, MSL2L1, RNF184

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23601-1-AP targets MSL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18727

  Product name: Recombinant human MSL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 228-577 aa of BC093790

  Sequence: TEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETVSNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSEKVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSHSKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC

• Full Name: male-specific lethal 2 homolog (Drosophila)
• Calculated Molecular Weight: 577 aa, 63 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC093790
• Gene Symbol: MSL2
• Gene ID (NCBI): 55167
• RRID: AB_3742087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9HCI7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammals, MSL2 participates in histone H2B ubiquitination (H2Bub1) via the MSL complex, regulating transcription and chromatin remodeling. It is essential for embryonic development and has been implicated in cancer progression. MSL2's dysfunction is linked to developmental defects and oncogenesis, highlighting its critical role in gene regulation. (PMID: 30669413; PMID: 34899909)