ChromoTek MSLN VHH, recombinant binding protein for 2x Cys conjugation

Validation Data Gallery

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  The affinity of anti-human MSLN recombinant VHH towards human MSLN was determined using biolayer interferometry (BLI). Biotinylated, recombinant human MSLN was immobilized on Streptavidin biosensors and assayed with 1.5 to 100 nM of FITC Plus conjugated-MSLN VHH (FITC-msln). Fit indicates a 1:1 binding model fitted to the data.

Alpaca anti-MSLN VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

Cat No. mslnCys2

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Synonyms

MSLN-Nanobody, MSLN-Single domain antibody (sdAb), CAK1 antigen, pre-pro-megakaryocyte-potentiating factor, MPF, MSLN, Mesothelin

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Product Information

Alpaca anti-MSLN VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

• Applications: Conjugation
• Reactivity: Human
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: Picomolar range, below the assay limit (biolayer interferometry)
• Molecular Weight: 15.710 kDa
• Form: Liquid
• RRID: AB_3101940
• Storage Buffer: 10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Background: Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

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