MSMP Polyclonal antibody

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  WB analysis of DU 145 using 19526-1-AP

  WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells.

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  WB analysis using 19526-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 19526-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MSMP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19526-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MSMP, PC 3, PC3 secreted microprotein, PSMP

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Tested Applications

• Positive WB detected in: PC-3 cells, DU 145 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19526-1-AP targets MSMP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microseminoprotein, prostate associated
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 12 kDa, 20 kDa
• GenBank Accession Number: NM_001044264
• Gene Symbol: MSMP
• Gene ID (NCBI): 692094
• RRID: AB_3085579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q1L6U9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.