Tested Applications
| Positive WB detected in | HT-1080 cells, human kidney tissue, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14547-1-AP targets MSRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6053 Product name: Recombinant human MSRA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC054033 Sequence: MLSATRRACQLLLLHSLFPVPRMGNSASNIVSPQEALPGRKEQTPVAAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLGGTGVSCPVGIKK Predict reactive species |
| Full Name | methionine sulfoxide reductase A |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 24-26 kDa |
| GenBank Accession Number | BC054033 |
| Gene Symbol | MSRA |
| Gene ID (NCBI) | 4482 |
| RRID | AB_2148366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJ68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MSRA antibody 14547-1-AP | Download protocol |
| IHC protocol for MSRA antibody 14547-1-AP | Download protocol |
| IP protocol for MSRA antibody 14547-1-AP | Download protocol |
| WB protocol for MSRA antibody 14547-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
14547-1-AP (anti-MsrA) has 18 citations across journals including Nature Cell Biology, Cell Reports, Free Radical Biology and Medicine, Antioxidants, Geroscience, Analytical Chemistry, and International Journal of Molecular Science. Research fields span oxidative stress, ferroptosis, aging/senescence, antioxidant signaling, methionine metabolism, cardiovascular disease, cancer, neurology, and food science. Applications include WB, IF, IP, and IHC across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) The Antioxidant Enzyme Methionine Sulfoxide Reductase A (MsrA) Interacts with Jab1/CSN5 and Regulates Its Function. | ||
Free Radic Biol Med Loss of methionine sulfoxide reductases increases resistance to oxidative stress. | ||
Oxid Med Cell Longev Fecal Microbiota Transplantation Is a Promising Method to Restore Gut Microbiota Dysbiosis and Relieve Neurological Deficits after Traumatic Brain Injury. | ||
Anal Chem Novel Visual Perspective for Tracking of Larval Development, Aging and Antiaging Drugs Screening by Fluorescent Sensing MsrA |
Reviews
What customers say
The single review for this product (used at 1:500 dilution on mouse liver tissue for Western Blot) rated it 4 out of 5 stars. The reviewer reported a clear band with a clean background, though they noted the signal was a little weak. Overall, the feedback is positive, indicating reliable specificity and low background, with only a minor concern about signal intensity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (12-01-2020) | Clear band with clean background. The signal is a little weak.
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