Tested Applications
| Positive WB detected in | HepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14682-1-AP targets MTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6400 Product name: Recombinant human MTA3 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-343 aa of BC053631 Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS Predict reactive species |
| Full Name | metastasis associated 1 family, member 3 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 62 kDa, 68 kDa |
| GenBank Accession Number | BC053631 |
| Gene Symbol | MTA3 |
| Gene ID (NCBI) | 57504 |
| RRID | AB_2298003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IHC protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IP protocol for MTA3 antibody 14682-1-AP | Download protocol |
| WB protocol for MTA3 antibody 14682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTA3 antibody (Cat# 14682-1-AP) has 19 total citations published across 18 journals, including Cell Stem Cell, Cancer Research, Molecular Cell, Nature Structural & Molecular Biology, Nucleic Acids Research, and Cell & Molecular Immunology. Associated research fields span cancer biology (colorectal, lung, liver, breast, prostate, and other carcinomas), chromatin remodeling via the NuRD complex, epigenetics, stem cell biology, and ferroptosis resistance.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis | ||
Cell Stem Cell The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. | ||
Cancer Res TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma | ||
Mol Cell The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. | ||
Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development | ||
Cancer Lett Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance |
