MTA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 14682-1-AP

Synonyms

mta1, MTA 3, Metastasis-associated protein MTA3

---

Validation Data Gallery

• 

  WB analysis using 14682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14682-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14682-1-AP

  IP result of anti-MTA3 (IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug.

• 

  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 9 publications below
• IP: See 1 publications below

Product Information

14682-1-AP targets MTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTA3 fusion protein Ag6400
• Full Name: metastasis associated 1 family, member 3
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 62 kDa, 68 kDa
• GenBank Accession Number: BC053631
• Gene Symbol: MTA3
• Gene ID (NCBI): 57504
• RRID: AB_2298003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell; The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development.; Authors - Jinzhong Qin
mouse		Mol Cell; The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.; Authors - Susanne Bornelöv
human	IHC	Oncotarget; Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis.; Authors - Yi Huang
human	WB,IHC	PLoS One; Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.; Authors - Li Haiying H ; KD Validated
human	IHC	J Gastroenterol Hepatol; Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma.; Authors - Chuanxi Wang
human	WB,IHC	Tumour Biol; MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway.; Authors - Taiwei Jiao