MTG1 Polyclonal antibody

MTG1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13742-1-AP

Synonyms

Mitochondrial ribosome-associated GTPase 1, Mitochondrial GTPase 1, GTPBP7, GTP-binding protein 7, GTP binding protein 7

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Validation Data Gallery

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  WB analysis of human brain using 13742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13742-1-AP (MTG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13742-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13742-1-AP (MTG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 13742-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 13742-1-AP (MTG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, human liver tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13742-1-AP targets MTG1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTG1 fusion protein Ag4681
• Full Name: mitochondrial GTPase 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 300 aa, 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC035721
• Gene Symbol: MTG1
• Gene ID (NCBI): 92170
• RRID: AB_2147380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang