MTHFD1 Monoclonal antibody

MTHFD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B1G3

Reviews (1)

Cat no : 67670-1-Ig

Synonyms

MTHFC, MTHFD, MTHFD1

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Validation Data Gallery

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  WB analysis using 67670-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 67670-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HEK-293 using 67670-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, A431 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

67670-1-Ig targets MTHFD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD1 fusion protein Ag24553
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Calculated molecular weight: 101 kDa
• GenBank accession number: BC009806
• Gene symbol: MTHFD1
• Gene ID (NCBI): 4522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Publications

Species	Application	Title
human	WB	Front Oncol; Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.; Authors - Changhong Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clean background. High dilution. There is an extra non-specific band around 70 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

MTHFD1 Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:67670-1-Ig)