Tested Applications
| Positive WB detected in | U2OS cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67670-1-Ig targets MTHFD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24553 Product name: Recombinant human MTHFD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 536-935 aa of BC009806 Sequence: TNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF Predict reactive species |
| Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC009806 |
| Gene Symbol | MTHFD1 |
| Gene ID (NCBI) | 4522 |
| RRID | AB_2882866 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTHFD1 antibody 67670-1-Ig | Download protocol |
| IHC protocol for MTHFD1 antibody 67670-1-Ig | Download protocol |
| WB protocol for MTHFD1 antibody 67670-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTHFD1 Monoclonal antibody (2B1G3), catalog number 67670-1-Ig, has 2 total citations. These citations appear in Journal of Biological Chemistry and Frontiers in Oncology. The research fields encompass mitochondrial folate metabolism in liver fibrosis resolution and RNA methylation-related gene biology in gallbladder carcinoma. Both studies utilized the antibody for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
J Biol Chem Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution
| ||
Front Oncol Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. |
Reviews
What customers say
The single review for this product (lot 10017178) was submitted by Hua Jiang on December 1, 2020, for Western Blot on mouse liver tissue at a 1:4000 dilution. The reviewer gave a 4-star rating, noting a clean background and good performance at high dilution, while mentioning an extra non-specific band around 70 kDa.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (12-01-2020) | Clean background. High dilution. There is an extra non-specific band around 70 kDa.
 |
