|Positive WB detected in||U2OS cells, A431 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67670-1-Ig targets MTHFD1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MTHFD1 fusion protein Ag24553|
|Full Name||methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase|
|Calculated molecular weight||101 kDa|
|GenBank accession number||BC009806|
|Gene ID (NCBI)||4522|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (12-01-2020)
Clean background. High dilution. There is an extra non-specific band around 70 kDa.