Tested Applications
| Positive WB detected in | A549 cells, HEK-293T cells, Jurkat cells, mouse testis tissue, rat testis tissue, HCT 116 cells |
| Positive IP detected in | A549 cells, mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12270-1-AP targets MTHFD2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2911 Product name: Recombinant human MTHFD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC017054 Sequence: MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN Predict reactive species |
| Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| Calculated Molecular Weight | 350 aa, 38 kDa |
| Observed Molecular Weight | 33-38 kDa |
| GenBank Accession Number | BC017054 |
| Gene Symbol | MTHFD2 |
| Gene ID (NCBI) | 10797 |
| RRID | AB_2147525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MTHFD2 antibody 12270-1-AP | Download protocol |
| IF protocol for MTHFD2 antibody 12270-1-AP | Download protocol |
| IHC protocol for MTHFD2 antibody 12270-1-AP | Download protocol |
| IP protocol for MTHFD2 antibody 12270-1-AP | Download protocol |
| WB protocol for MTHFD2 antibody 12270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTHFD2 Polyclonal Antibody (Cat# 12270-1-AP) has 67 citations spanning journals including Nature, Science, Cell Metabolism, Nature Communications, Molecular Cell, Science Advances, EMBO Journal, and Cell Reports. Research fields cover one-carbon/folate metabolism, mitochondrial biology, cancer metabolic reprogramming, oxidative stress and redox signaling, fibrosis, autoimmune disease, cardiomyopathy, and neurodegeneration. Cited applications are predominantly Western blot, with IHC, IF, IP, and Co-IP also represented.
| Species | Application | Title |
|---|---|---|
Cancer Cell Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. | ||
Science mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. | ||
Cell Metab mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression. |
Reviews
What customers say
Across three reviews, this MTHFD2 antibody shows mixed performance in Western blot. One user reported excellent results with no background in mouse hepatoma lysates at 1:1000. Another observed the correct band in mouse satellite cells at 1:2000 but noted some non-specific bands below 30 kDa. A third user experienced no band at the expected size in mouse liver lysate at 1:500, instead seeing a strong non-specific band at 100 kDa. Overall, results appear system- and dilution-dependent, with optimal performance at higher dilutions in specific tissue contexts.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (10-17-2024) | I referred to the following publication when looking for an MTHFD2 antibody used for WB. Cancer Cell . 2012 Jul 10;22(1):66-79. The data in the paper is very convincing to me, so I ordered the antibody and tested it myself in mouse hepatomas. The antibody worked great without any background.
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Ayse Tarbin (Verified Customer) (03-16-2023) | It was a sample gift antibody. It has a band at corresponding kDa but there are also unspesific bands just bellow 30 kDa.
 |
Hua (Verified Customer) (10-21-2020) | There is no band at the expected size. There is a strong non-specific band at 100 kDa.
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