MTHFD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTHFD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

45

Reviews (3)

Cat no : 12270-1-AP

Synonyms

Methenyltetrahydrofolate cyclohydrolase, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

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Validation Data Gallery

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  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:4000) with A549 cells lysate 1480 ug.

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  IP experiment of mouse testis using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (12270-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, Jurkat cells, mouse testis tissue, rat testis tissue, HCT 116 cells
• Positive IP detected in: A549 cells, mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 40 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12270-1-AP targets MTHFD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTHFD2 fusion protein Ag2911
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC017054
• Gene Symbol: MTHFD2
• Gene ID (NCBI): 10797
• RRID: AB_2147525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

Publications

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova
mouse	WB	Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
mouse	WB	Cell Metab; Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.; Authors - Sukru Anil Dogan
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jennifer (Verified Customer) (10-17-2024)

I referred to the following publication when looking for an MTHFD2 antibody used for WB. Cancer Cell . 2012 Jul 10;22(1):66-79. The data in the paper is very convincing to me, so I ordered the antibody and tested it myself in mouse hepatomas. The antibody worked great without any background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse hepatomas.

FH

Ayse Tarbin (Verified Customer) (03-16-2023)

It was a sample gift antibody. It has a band at corresponding kDa but there are also unspesific bands just bellow 30 kDa.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:2000 in 5% milk in TBST
• Cell Tissue Type: Mouse satellite cells

MTHFD2 Antibody Western Blot,Cell culture validation (1:2000 in 5% milk in TBST dilution) in Mouse satellite cells (Cat no:12270-1-AP)

FH

Hua (Verified Customer) (10-21-2020)

There is no band at the expected size. There is a strong non-specific band at 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver lysate

MTHFD2 Antibody Western Blot validation (1:500 dilution) in mouse liver lysate (Cat no:12270-1-AP)