Tested Applications
| Positive WB detected in | L02 cells, Jurkat cells |
| Positive IHC detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13924-1-AP targets MTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4940 Product name: Recombinant human MTM1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-362 aa of BC030779 Sequence: MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGATSRGENSYGLDITCKDMRNLRFALKQEGHSRRDMFEILTRYAFPLAHSLPLFAFLNEEKFNVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLTIYDARPSVNAVANKATGGGYESDDAYHNAELFFLDIHNIHVMRESLKKVKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQVAD Predict reactive species |
| Full Name | myotubularin 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC030779 |
| Gene Symbol | MTM1 |
| Gene ID (NCBI) | 4534 |
| RRID | AB_2147700 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13496 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The MTM1(Myotubularin) gene encodes a protein that belongs to a family of putative tyrosine phosphatases. It is required for muscle cell differentiation. The protein is also a potent phosphatidylinositol 3-phosphate (PI3P) phosphatase, which is a 66 kDa ubiquitous protein that is found mutated in the X-linked genetic disorder myotubular myopathy(XLMTM).MTM1 could therefore act as a regulator of the highly active phosphoinositide (PI) metabolism in cells, where interconversions of the eight PIs described so far is controlled by a set of specific kinases and phosphatases(PMID:14660569). MTM1 has two isoforms with the molecular mass of 66 kDa and 70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTM1 antibody 13924-1-AP | Download protocol |
| IHC protocol for MTM1 antibody 13924-1-AP | Download protocol |
| WB protocol for MTM1 antibody 13924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTM1 Polyclonal antibody (Cat# 13924-1-AP) has 3 citations. They appear in Nature Communications (neuronal autophagy and mitochondrial damage), Pharmaceuticals (Basel) (hepatocellular carcinoma lncRNA signature and triaptosis), and PLoS Pathogens (pseudorabies virus entry and TRPML1 regulation). The research fields span neurobiology, oncology, and virology, with applications in WB across human, mouse, and pig species.
| Species | Application | Title |
|---|---|---|
Nat Commun Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy. | ||
Pharmaceuticals (Basel) From Single-Cell and Bulk Transcriptomic Integration to Functional Verification: Triaptosis-Associated lncRNA Signature Predicts Survival and Guides Therapy in Hepatocellular Carcinoma.
| ||
PLoS Pathog Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry |
Reviews
What customers say
The single review for 13924-1-AP is positive, rated 5 stars. The reviewer used the antibody for Western Blot at a 1:1000 dilution on mouse heart tissue and reported that it worked well. Overall, the feedback indicates reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Siting (Verified Customer) (01-28-2020) | This antibody worked for western blot.
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