MTMR14 Monoclonal antibody

MTMR14 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A8B2

Cat no : 67561-1-Ig

Synonyms

C3orf29, FLJ22405, FLJ46453, FLJ90311, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

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Validation Data Gallery

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  WB analysis using 67561-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67561-1-Ig (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 67561-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67561-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 67561-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MTMR14 antibody (67561-1-Ig, Clone: 1A8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67561-1-Ig targets MTMR14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTMR14 fusion protein Ag6883
• Full Name: myotubularin related protein 14
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC050626
• Gene Symbol: MTMR14
• Gene ID (NCBI): 64419
• RRID: AB_2882775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; MTMR14 Alleviates Chronic Obstructive Pulmonary Disease as a Regulator in Inflammation and Emphysema.; Authors - Yiya Gu
mouse,rat	IF	J Am Heart Assoc; Myotubularin-Related Protein14 Prevents Neointima Formation and Vascular Smooth Muscle Cell Proliferation by Inhibiting Polo-Like Kinase1; Authors - Ling-Yao Kong