MTMR14 Monoclonal antibody

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• MTMR14 Monoclonal antibody (67561-1-Ig)

MTMR14 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67561-1-Ig Copied!

Clone No. 1A8B2

Publications (2)

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Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

C3orf29, EC:3.1.3.-, HCV NS5A-transactivated protein 4 splice variant A-binding protein 1, hEDTP, hJumpy

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  WB analysis using 67561-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67561-1-Ig (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse heart using 67561-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 67561-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse heart using 67561-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MTMR14 antibody (67561-1-Ig, Clone: 1A8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67561-1-Ig targets MTMR14 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6883

  Product name: Recombinant human MTMR14 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 245-598 aa of BC050626

  Sequence: LSIPYPGCEFFKEYKDRDYMAEGLIFNWKQDYVDAPLSIPDFLTHSLNIDWSQYQCWDLVQQTQNYLKLLLSLVNSDDDSGLLVHCISGWDRTPLFISLLRLSLWADGLIHTSLKPTEILYLTVAYDWFLFGHMLVDRLSKGEEIFFFCFNFLKHITSEEFSALKTQRRKSLPARDGGFTLEDICMLRRKDRGSTTSLGSDFSLVMESSPGATGSFTYEAVELVPAGAPTQAAWRKSHSSSPQSVLWNRPQPSEDRLPSQQGLAEARSSSSSSSNHSDNFFRMGSSPLEVPKPRLAALSDRETRLQEVRSAFLAAYSSTVGLRAVAPSPSGAIGGLLEQFARGVGLRSISSNAL

• Full Name: myotubularin related protein 14
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC050626
• Gene Symbol: MTMR14
• Gene ID (NCBI): 64419
• RRID: AB_2882775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NCE2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.

Publications

What published studies show

The MTMR14 Polyclonal Antibody (Cat# 67561-1-Ig) has 2 total citations. These appear in Oxidative Medicine and Cellular Longevity and Journal of the American Heart Association. The associated research fields are pulmonology (COPD, inflammation, emphysema) and cardiovascular biology (neointima formation, vascular smooth muscle cell proliferation). Applications cited include Western Blot and Immunofluorescence in human, rat, and mouse samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oxid Med Cell Longev; MTMR14 Alleviates Chronic Obstructive Pulmonary Disease as a Regulator in Inflammation and Emphysema.; Authors - Yiya Gu
rat,mouse	IF	J Am Heart Assoc; Myotubularin-Related Protein14 Prevents Neointima Formation and Vascular Smooth Muscle Cell Proliferation by Inhibiting Polo-Like Kinase1; Authors - Ling-Yao Kong