MTMR14 Monoclonal antibody, PBS Only

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  WB analysis using 67561-1-Ig (same clone as 67561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67561-1-Ig (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MTMR14 antibody (67561-1-Ig, Clone: 1A8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

MTMR14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67561-1-PBS

Clone No.1A8B2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C3orf29, FLJ22405, FLJ46453, FLJ90311, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

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Product Information

67561-1-PBS targets MTMR14 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6883

  Product name: Recombinant human MTMR14 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 245-598 aa of BC050626

  Sequence: LSIPYPGCEFFKEYKDRDYMAEGLIFNWKQDYVDAPLSIPDFLTHSLNIDWSQYQCWDLVQQTQNYLKLLLSLVNSDDDSGLLVHCISGWDRTPLFISLLRLSLWADGLIHTSLKPTEILYLTVAYDWFLFGHMLVDRLSKGEEIFFFCFNFLKHITSEEFSALKTQRRKSLPARDGGFTLEDICMLRRKDRGSTTSLGSDFSLVMESSPGATGSFTYEAVELVPAGAPTQAAWRKSHSSSPQSVLWNRPQPSEDRLPSQQGLAEARSSSSSSSNHSDNFFRMGSSPLEVPKPRLAALSDRETRLQEVRSAFLAAYSSTVGLRAVAPSPSGAIGGLLEQFARGVGLRSISSNAL

• Full Name: myotubularin related protein 14
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC050626
• Gene Symbol: MTMR14
• Gene ID (NCBI): 64419
• RRID: AB_2882775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NCE2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.