Tested Applications
| Positive WB detected in | MCF-7 cells, human liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15650-1-AP targets MTO1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8192 Product name: Recombinant human MTO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 304-653 aa of BC005808 Sequence: LEKAKVIQPDGVLLLLPRMECNGAISAHHNLPLPGYGVQYDYLDPRQITPSLETHLVQRLFFAGQINGTTGYEEAAAQGVIAGINASLRVSRKPPFVVSRTEGYIGVLIDDLTTLGTSEPYRMFTSRVEFRLSLRPDNADSRLTLRGYKDAGCVSQQRYERACWMKSSLEEGISVLKSIEFLSSKWKKLIPEASISTSRSLPVRALDVLKYEEVDMDSLAKAVPEPLKKYTKCRELAERLKIEATYESVLFHQLQEIKGVQQDEALQLPKDLDYLTIRDVSLSHEVREKLHFSRPQTIGAASRIPGVTPAAIINLLRFVKTTQRRQSAMNESSKTDQYLCDADRLQEREL Predict reactive species |
| Full Name | mitochondrial translation optimization 1 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 717 aa, 80 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC005808 |
| Gene Symbol | MTO1 |
| Gene ID (NCBI) | 25821 |
| RRID | AB_2266805 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Z2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTO1 antibody 15650-1-AP | Download protocol |
| IHC protocol for MTO1 antibody 15650-1-AP | Download protocol |
| WB protocol for MTO1 antibody 15650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody 15650-1-AP (anti-MTO1) has 12 total citations across journals including Nature Communications, Nucleic Acids Research, American Journal of Human Genetics, Human Molecular Genetics, Journal of Hepatology, Scientific Reports, Biochimica et Biophysica Acta, Journal of Proteome Research, and Molecular Biology Reports. Research fields span mitochondrial tRNA modification, mitochondrial diseases (MELAS, cardiomyopathy), metabolic reprogramming, oxidative phosphorylation, and cancer biology (melanoma, ovarian cancer). Applications are predominantly WB and CoIP in human samples.
| Species | Application | Title |
|---|---|---|
J Hepatol Severe respiratory complex III defect prevents liver adaptation to prolonged fasting. | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
Nucleic Acids Res Restoration of mitochondrial function through activation of hypomodified tRNAs with pathogenic mutations associated with mitochondrial diseases | ||
Nucleic Acids Res The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. | ||
Am J Hum Genet Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis. | ||
Sci Rep microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases. |
