Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue, rat liver tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14257-1-AP targets MTP18 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5530 Product name: Recombinant human MTP18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC038831 Sequence: MSEPQPRGAERDLYRDTWVRYLGYANEVGEAFRSLVPAAVVWLSYGVASSYVLADAIDKGKKAGEVPSPEAGRSARVTVAVVDTFVWQALASVAIPGFTINRVCAASLYVLGTATRWPLAVRKWTTTALGLLTIPIIIHPIDRSVDFLLDSSLRKLYPTVGKPSSS Predict reactive species |
| Full Name | mitochondrial protein 18 kDa |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC038831 |
| Gene Symbol | MTP18 |
| Gene ID (NCBI) | 51537 |
| RRID | AB_2148123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UDX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitochondrial 18 kDa protein (MTP18), also designated as Mitochondrial Fission Process 1 (MTFP1), is a nuclear-encoded mitochondrial inner membrane protein that functions as a critical regulator of mitochondrial dynamics and cell viability. Perturbations in MTP18 expression have significant consequences for cell fate: knockdown leads to cytochrome c release and apoptosis, while its overexpression is linked to excessive mitochondrial fragmentation in pathological contexts such as chemotherapy-induced cardiotoxicity and gastric cancer cell apoptosis. Collectively, MTP18 serves as a multifunctional mitochondrial effector that integrates phosphoinositide signaling, nutrient sensing, and membrane remodeling to govern mitochondrial morphology, quality control, and cell survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MTP18 antibody 14257-1-AP | Download protocol |
| IP protocol for MTP18 antibody 14257-1-AP | Download protocol |
| WB protocol for MTP18 antibody 14257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MTFP1 antibody (Cat# 14257-1-AP) has 7 citations spanning journals including Cell, Cell Death & Disease, Journal of Translational Medicine, Journal of Cell Biology, Frontiers in Cellular Neuroscience, Current Issues in Molecular Biology, and Cell Biology International. Research fields covered include mitochondrial fission/fusion dynamics, hepatocellular carcinoma, oral squamous cell carcinoma, acute lung injury, myocardial infarction, and neurodegeneration. Applications used were primarily Western blot and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels
| ||
Cell Death Dis MTP18 overexpression contributes to tumor growth and metastasis and associates with poor survival in hepatocellular carcinoma. | ||
J Transl Med TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury | ||
J Cell Biol Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling. | ||
Front Cell Neurosci Reduced mitochondrial size in hippocampus and psychiatric behavioral changes in the mutant mice with homologous mutation of Timm8a1-I23fs49X | ||
Curr Issues Mol Biol Identification and Validation of MTFP1 as a Mitochondrial Target Restoring Dynamics and ECM Remodeling in Acute Myocardial Infarction. |
