MTP18 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTP18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14257-1-AP

Synonyms

HSPC242, Mitochondrial 18 kDa protein, mitochondrial protein 18 kDa, MTFP1, MTP18, My022

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 14257-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14257-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14257-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14257-1-AP (MTP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 14257-1-AP

  IP result of anti-MTP18 (IP:14257-1-AP, 4ug; Detection:14257-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 14257-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14257-1-AP (MTP18 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 14257-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14257-1-AP (MTP18 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

14257-1-AP targets MTP18 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTP18 fusion protein Ag5530
• Full Name: mitochondrial protein 18 kDa
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC038831
• Gene Symbol: MTP18
• Gene ID (NCBI): 51537
• RRID: AB_2148123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.; Authors - Hidenori Otera
human	IHC	Cell Death Dis; MTP18 overexpression contributes to tumor growth and metastasis and associates with poor survival in hepatocellular carcinoma.; Authors - Yu Zhang
human	WB	Cell Biol Int; MTFP1 overexpression promotes the growth of oral squamous cell carcinoma by inducing ROS production.; Authors - Tingying Xiao ; KD Validated
mouse	WB	Front Cell Neurosci; Reduced mitochondrial size in hippocampus and psychiatric behavioral changes in the mutant mice with homologous mutation of Timm8a1-I23fs49X; Authors - Niemtiah Ouattara
mouse	WB	J Transl Med; TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury; Authors - Wen-Jing Zhong
human	WB	Cell; MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels; Authors - Luis Carlos Tábara ; KO Validated