MUC1/CA15-3 Monoclonal antibody

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  IHC staining of human rectal cancer using 60749-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60749-1-Ig (MUC1/CA15-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 60749-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60749-1-Ig (CA15-3,MUC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60749-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60749-1-Ig (MUC1/CA15-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MUC1/CA15-3 Monoclonal Antibody for IHC, ELISA

Cat No. 60749-1-Ig

Clone No.1D5D11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

CA15-3, CA153, MUC1, CA15 3, CA 15-3

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Tested Applications

• Positive IHC detected in: human rectal cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1600-1:6400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60749-1-Ig targets MUC1/CA15-3 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MUC1/CA15-3 fusion protein Ag17660
• Full Name: mucin 1, cell surface associated
• Calculated Molecular Weight: 1264 aa, 123 kDa
• GenBank Accession Number: BC120975
• Gene Symbol: MUC1/CA15-3
• Gene ID (NCBI): 4582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P15941
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas.. Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies.