MUC20 Polyclonal antibody, PBS Only

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• MUC20 Polyclonal antibody, PBS Only (13380-1-PBS)

• 

  WB analysis of human liver using 13380-1-AP (same clone as 13380-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 13380-1-AP (same clone as 13380-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13380-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 13380-1-AP (same clone as 13380-1-PBS)

  IP result of anti-MUC20 (IP:13380-1-AP, 4ug; Detection:13380-1-AP 1:300) with mouse liver tissue lysate 6000 ug. This data was developed using the same antibody clone with 13380-1-PBS in a different storage buffer formulation.

MUC20 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 13380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

13380-1-PBS targets MUC20 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4205

  Product name: Recombinant human MUC20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 255-555 aa of BC029267

  Sequence: TLLAEALVTVTNIEVINCSITEIETTTSSIPGASDTDPIPTEGVKASSTSDPPALPDSTEAKPHITEVTASAETLSTAGTTESAAPDATIGTPLPTNSTIEREVTAPGATTLSGALATGNPLEETSALSVETPSYVKVSGAAPVSIEAGSAVGKTTSFAGSSASSYSPSEAALKNFTPSETLTTDIATKGPFPTSRDPLPSVPPTTTNSSRGTNSTLAKITTSAKTTMKPPTATPTTARTRPTTDMSAGENGGFLLLRLSVASPEDLTDPRVAERLMQQLHRELHAHAPHFQVSLLRVRRG

• Full Name: mucin 20, cell surface associated
• Calculated Molecular Weight: 55 kDa, 71 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC029267
• Gene Symbol: MUC20
• Gene ID (NCBI): 200958
• RRID: AB_2235526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N307
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).