Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84879-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Methylmalonyl Coenzyme A mutase/MUTantibody (84879-6-RR, Clone: 242198A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84879-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using 84879-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Methylmalonyl Coenzyme A mutase/MUT Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 84879-6-RR

Clone No.242198A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

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Tested Applications

• Positive IHC detected in: Human Hepatocellular cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A431 cells, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84879-6-RR targets Methylmalonyl Coenzyme A mutase/MUT in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Full Name: methylmalonyl Coenzyme A mutase
• Calculated Molecular Weight: 750 aa, 83 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC016282
• Gene Symbol: MUT
• Gene ID (NCBI): 4594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P22033
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.