MVD Polyclonal antibody, PBS Only

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• MVD Polyclonal antibody, PBS Only (15331-1-PBS)

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  WB analysis using 15331-1-AP (same clone as 15331-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15331-1-PBS

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15331-1-AP (same clone as 15331-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15331-1-AP (same clone as 15331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15331-1-AP (same clone as 15331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15331-1-AP (MVD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15331-1-AP (same clone as 15331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15331-1-AP (same clone as 15331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 15331-1-AP (same clone as 15331-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MVD antibody (15331-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 15331-1-AP (same clone as 15331-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MVD antibody (15331-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15331-1-PBS in a different storage buffer formulation.

MVD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15331-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

diphospho, EC:4.1.1.33, Mevalonate, MPD

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Product Information

15331-1-PBS targets MVD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7255

  Product name: Recombinant human MVD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC000011

  Sequence: MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA

• Full Name: mevalonate (diphospho) decarboxylase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 66-74 kDa, 45 kDa, 37 kDa
• GenBank Accession Number: BC000011
• Gene Symbol: MVD
• Gene ID (NCBI): 4597
• RRID: AB_2147439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53602
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).