Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12360-1-AP targets MXI1 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3024 Product name: Recombinant human MXI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC012907 Sequence: MPSPRLQHSKPPRRLSRAQKHSSGSSNTSTANRRAHLRLCLERLKVLIPLGPDCTRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEMERIRMDSIGSTISSDRSDSEREEIEVDVESTEFSHGEVDNISTTSISDIDDHSSLPSIGSDEGYSSASVKLSFTS Predict reactive species |
| Full Name | MAX interactor 1 |
| Calculated Molecular Weight | 228 aa, 26 kDa |
| Observed Molecular Weight | 26-29 kDa |
| GenBank Accession Number | BC012907 |
| Gene Symbol | MXI1 |
| Gene ID (NCBI) | 4601 |
| RRID | AB_2877848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50539 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MXI1 antibody 12360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MXI1 Polyclonal antibody (Cat# 12360-1-AP) has 3 total citations. These appear in Nature Communications, Cancer Immunology & Immunotherapy, and Frontiers in Molecular Biosciences. The associated research fields include clear cell renal cell carcinoma (epigenetic and transcriptomic characterization), acute megakaryoblastic leukemia (MXI1-mediated MAPK signaling), and osteosarcoma (hypoxic gene signature for prognosis and chemosensitivity prediction). Applications cited are WB and ChIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Epigenetic and transcriptomic characterization reveals progression markers and essential pathways in clear cell renal cell carcinoma | ||
Cancer Immunol Immunother FUBP3 mediates MXI1 stability to silence RRAS and hinder MAPK signaling in acute megakaryoblastic leukemia progression. | ||
Front Mol Biosci Development and Verification of a Hypoxic Gene Signature for Predicting Prognosis, Immune Microenvironment, and Chemosensitivity for Osteosarcoma. |
Reviews
What customers say
The product has one user review. A researcher named Vincenzo Tarallo rated it 4 out of 5 stars for Western Blot application (lot 00011183), commenting that the antibody works well for blotting. The review was posted on August 7, 2020. Overall, the feedback is positive, with the user confirming good performance in their intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vincenzo (Verified Customer) (08-07-2020) | Antibody works well for blotting
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