MXI1 Polyclonal antibody

MXI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 12360-1-AP

Synonyms

Max-interacting protein 1, MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 12360-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12360-1-AP (MXI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12360-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12360-1-AP (MXI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12360-1-AP targets MXI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MXI1 fusion protein Ag3024
• Full Name: MAX interactor 1
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC012907
• Gene Symbol: MXI1
• Gene ID (NCBI): 4601
• RRID: AB_2877848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.

Publications

Species	Application	Title
human	WB	Front Mol Biosci; Development and Verification of a Hypoxic Gene Signature for Predicting Prognosis, Immune Microenvironment, and Chemosensitivity for Osteosarcoma.; Authors - Fengfeng Wu
human	WB	Nat Commun; Epigenetic and transcriptomic characterization reveals progression markers and essential pathways in clear cell renal cell carcinoma; Authors - Yige Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vincenzo (Verified Customer) (08-07-2020)

Antibody works well for blotting

• Applications: Western Blot