Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse pancreas tissue |
| Positive IHC detected in | human skeletal muscle tissue, human bladder tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | myosin binding protein C, slow type |
| Calculated Molecular Weight | 128 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | NM_002465 |
| Gene Symbol | MYBPC1 |
| Gene ID (NCBI) | 4604 |
| RRID | AB_11182592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00872 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| IHC protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. | ||
Dokl Biochem Biophys Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains? | ||
Adv Biol (Weinh) Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sirsendu (Verified Customer) (06-19-2020) | Used for immunohistochemistry of db/db mice skin tissue after wound healing.Please provide amazon UK gift (£20)
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