MYBPC1 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 22900-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

MYBPC1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22900-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C-protein, skeletal muscle slow isoform, MYBPCS, Myosin-binding protein C, slow-type, Slow MyBP C, Slow MyBP-C

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse pancreas tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human bladder tissue, human heart tissue, human skeletal muscle tissue, mouse bladder tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin binding protein C, slow type
• Calculated Molecular Weight: 128 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: NM_002465
• Gene Symbol: MYBPC1
• Gene ID (NCBI): 4604
• RRID: AB_11182592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00872
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	IHC	Mol Psychiatry; FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors.; Authors - Minjie Shen
rat	WB	Dokl Biochem Biophys; Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?; Authors - I O Petrova
mouse	IHC	Adv Biol (Weinh); Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System; Authors - Mangesh Morey

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sirsendu (Verified Customer) (06-19-2020)

Used for immunohistochemistry of db/db mice skin tissue after wound healing.Please provide amazon UK gift (£20)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mice skin tissue