Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse pancreas tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human bladder tissue, human heart tissue, human skeletal muscle tissue, mouse bladder tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin binding protein C, slow type |
| Calculated Molecular Weight | 128 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | NM_002465 |
| Gene Symbol | MYBPC1 |
| Gene ID (NCBI) | 4604 |
| RRID | AB_11182592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00872 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| WB protocol for MYBPC1 antibody 22900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYBPC1 Polyclonal antibody (Cat# 22900-1-AP) has 3 total citations. These appear in Molecular Psychiatry (schizophrenia/neuroscience), Advanced Biology (nucleic acid delivery/biomaterials), and Doklady of Biochemistry and Biophysics (muscle physiology). Applications cited include IHC and WB across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. | ||
Adv Biol (Weinh) Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System | ||
Dokl Biochem Biophys Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains? |
Reviews
What customers say
The single review for 22900-1-AP is a 5-star rating. The reviewer used the antibody for immunohistochemistry on db/db mouse skin tissue following wound healing, at a 1:200 dilution. Overall, the feedback is positive, indicating the product performed well in this IHC application on mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sirsendu (Verified Customer) (06-19-2020) | Used for immunohistochemistry of db/db mice skin tissue after wound healing.Please provide amazon UK gift (£20)
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