MYBPC3 Polyclonal antibody, PBS Only

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• MYBPC3 Polyclonal antibody, PBS Only (19977-1-PBS)

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  WB analysis using 19977-1-AP (same clone as 19977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 19977-1-AP (same clone as 19977-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 19977-1-AP (same clone as 19977-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

MYBPC3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19977-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3, Myosin-binding protein C, cardiac-type

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Product Information

19977-1-PBS targets MYBPC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin binding protein C, cardiac
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141-150 kDa
• GenBank Accession Number: NM_000256
• Gene Symbol: MYBPC3
• Gene ID (NCBI): 4607
• RRID: AB_10858232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14896
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)