Filter: AllIHCIF/ICCFC (Intra)

IHC staining of human brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 27951-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of U2OS using 27951-1-AP
1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYCBP2 Polyclonal antibody

Name: MYCBP2 Polyclonal antibody
Brand: Proteintech
SKU: 27951-1-AP

MYCBP2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 27951-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KIAA0916, MYC binding protein 2, PAM, Pam/highwire/rpm 1 protein, Protein associated with Myc

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in	human brain tissue, rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells
Positive FC (Intra) detected in	U2OS cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 2 publications below

Product Information

27951-1-AP targets MYCBP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27228 Product name: Recombinant human MYCBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2672-2880 aa of NM_015057 Sequence: MGNSKGDRGNISTSSKPASTSGKSELSSKHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSRSLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSGASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPKSVPKDSTDSPGSENRA Predict reactive species
Full Name	MYC binding protein 2
Calculated Molecular Weight	510 kDa
GenBank Accession Number	NM_015057
Gene Symbol	MYCBP2
Gene ID (NCBI)	23077
RRID	AB_2881020
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75592
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for MYCBP2 antibody 27951-1-AP	Download protocol
IHC protocol for MYCBP2 antibody 27951-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Front Immunol MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients Authors - Guilin Wang View Article
human	IHC	Arch Biochem Biophys Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics Authors - Ming-Ying Ling View Article