MYH1 Monoclonal antibody

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  WB analysis of human skeletal muscle using 67299-1-Ig

  1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig skeletal muscle using 67299-1-Ig

  1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 67299-1-Ig

  1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 67299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

MYH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67299-1-Ig

Clone No.1G10H9

Publications(10)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

1G10H9, MyHC-2x, MyHC-IIx/d, Myosin 1, Myosin-1

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, pig skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

67299-1-Ig targets MYH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17129

  Product name: Recombinant human MYH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC114545

  Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV

• Full Name: myosin, heavy chain 1, skeletal muscle, adult
• Calculated Molecular Weight: 1939 aa, 223 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC114545
• Gene Symbol: MYH1
• Gene ID (NCBI): 4619
• RRID: AB_2882563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12882
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	BMC Genomics; Identification of different myofiber types in pigs muscles and construction of regulatory networks; Authors - Chenchen Li
pig	WB	Food Funct; Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.; Authors - Lingyu Zhang
human	IHC	Pathol Res Pract; Layer-specific proteomic profiling of human normal heart; Authors - Yu Kakimoto
mouse	IF	Nutrients; Sodium Butyrate Alleviates Heat Stress-Induced Oxidative Stress and Skeletal Muscle Homeostasis Disruption by Promoting Autophagy in Mice; Authors - Jiayin Lu
rabbit	IHC	Invest Ophthalmol Vis Sci; One-Month-Old Rabbits Exhibit a Longer Postoperative Remodeling in Extraocular Muscles Compared to 3-Month-Old Rabbits; Authors - Jiangtao Lou
mouse	WB	J Muscle Res Cell Motil; RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging; Authors - Runqing Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moon (Verified Customer) (10-10-2022)

normal tissue-1/2 (N1/N2) tumor tissue-1/2/3 (T1/T2/T3)

• Applications: Western Blot
• Primary Antibody Dilution: 1:100000
• Cell Tissue Type: renal carcinoma cells

MYH1 Antibody Western Blot validation (1:100000 dilution) in renal carcinoma cells (Cat no:67299-1-Ig)