Validation Data Gallery
|Positive WB detected in||rabbit stomach tissue, human ileum tissue, rat rectum tissue, HeLa cells, rabbit large intestine, pig stomach, pig rectum, rat colon, mouse colon|
|Positive IHC detected in||human breast hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60222-1-Ig is SMMHC Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig, rabbit|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SMMHC fusion protein Ag13397|
|Full Name||myosin, heavy chain 11, smooth muscle|
|Calculated molecular weight||1938 aa, 224 kDa|
|Observed molecular weight||220 kDa|
|GenBank accession number||BC101677|
|Gene ID (NCBI)||4629|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.
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