SMMHC Monoclonal antibody

SMMHC Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5C6D3

Cat no : 60222-1-Ig

Synonyms

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain



Tested Applications

Positive WB detected inhuman ileum tissue, HeLa cells, mouse colon tissue, pig colon tissue, rat colon tissue, rat small intestine tissue
Positive IHC detected inhuman breast hyperplasia tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 60222-1-Ig is SMMHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMMHC fusion protein Ag13397
Full Name myosin, heavy chain 11, smooth muscle
Calculated molecular weight 1938 aa, 224 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC101677
Gene symbol MYH11
Gene ID (NCBI) 4629
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Protocols

Product Specific Protocols
WB protocol for SMMHC antibody 60222-1-IgDownload protocol
IHC protocol for SMMHC antibody 60222-1-IgDownload protocol
IF protocol for SMMHC antibody 60222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nutr Metab Cardiovasc Dis

Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.

Authors - Qiao Yu