SMMHC Monoclonal antibody

SMMHC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig, rabbit







Cat no : 60222-1-Ig


MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

Tested Applications

Positive WB detected inrabbit stomach tissue, human ileum tissue, rat rectum tissue, HeLa cells, rabbit large intestine, pig stomach, pig rectum, rat colon, mouse colon
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60222-1-Ig targets SMMHC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMMHC fusion protein Ag13397
Full Name myosin, heavy chain 11, smooth muscle
Calculated Molecular Weight 1938 aa, 224 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC101677
Gene Symbol MYH11
Gene ID (NCBI) 4629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.


Product Specific Protocols
WB protocol for SMMHC antibody 60222-1-IgDownload protocol
IHC protocol for SMMHC antibody 60222-1-IgDownload protocol
IF protocol for SMMHC antibody 60222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Basis Dis

Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.

Authors - Bao-Ying Luo


Nrf2 deficiency attenuates atherosclerosis by reducing LOX-1-mediated proliferation and migration of vascular smooth muscle cells.

Authors - Hongliang Li

Nutr Metab Cardiovasc Dis

Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.

Authors - Qiao Yu

Int J Mol Sci

Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells

Authors - Yiwen Zha

Mol Cell Biochem

HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress

Authors - Linli Wang