Filter: AllWBIHC

WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
WB analysis of human ileum using 60222-1-Ig (same clone as 60222-1-PBS)
human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.
IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

SMMHC Monoclonal antibody, PBS Only

SMMHC Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60222-1-PBS

Clone No.5C6D3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Synonyms

MYH11, 5C6D3, KIAA0866, Myosin 11, Myosin heavy chain 11

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60222-1-PBS targets SMMHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag13397 Product name: Recombinant human MYH11 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC101677 Sequence: MAQKGQLSDDEKFLFVDKNFINSPVAQADWAAKRLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDTSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVEAMAIMGFSEEEQLSILKVVS Predict reactive species
Full Name	myosin, heavy chain 11, smooth muscle
Calculated Molecular Weight	1938 aa, 224 kDa
Observed Molecular Weight	220 kDa
GenBank Accession Number	BC101677
Gene Symbol	SMMHC/MYH11
Gene ID (NCBI)	4629
RRID	AB_11182594
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P35749
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.