MYH14 Monoclonal antibody

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  WB analysis of COLO 320 using 66825-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66825-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 66825-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

MYH14 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66825-1-Ig

Clone No.1B4C9

Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C

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Tested Applications

• Positive WB detected in: COLO 320 cells, HT-29 cells
• Positive IHC detected in: human colon tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:100000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Product Information

66825-1-Ig targets MYH14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28063

  Product name: Recombinant human MYH14 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1231-1430 aa of NM_001077186

  Sequence: MQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTE

• Full Name: myosin, heavy chain 14
• Calculated Molecular Weight: 228 kDa
• Observed Molecular Weight: 200-230 kDa
• GenBank Accession Number: NM_001077186
• Gene Symbol: MYH14
• Gene ID (NCBI): 79784
• RRID: AB_2882168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z406
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation; Authors - Helena Sofia Domingues ; KD Validated
	IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.; Authors - Helena Sofia Domingues