MYH14 Monoclonal antibody, PBS Only

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  WB analysis of COLO 320 using 66825-1-Ig (same clone as 66825-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66825-1-Ig (same clone as 66825-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MYH14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66825-1-PBS

Clone No.1B4C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

myosin, MHC16, KIAA2034, FP17425, DFNA4

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Product Information

66825-1-PBS targets MYH14 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28063

  Product name: Recombinant human MYH14 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1231-1430 aa of NM_001077186

  Sequence: MQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTE

• Full Name: myosin, heavy chain 14
• Calculated Molecular Weight: 228 kDa
• Observed Molecular Weight: 200-230 kDa
• GenBank Accession Number: NM_001077186
• Gene Symbol: MYH14
• Gene ID (NCBI): 79784
• RRID: AB_2882168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z406
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).