MYH2-specific Polyclonal antibody

MYH2-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55069-1-AP

Synonyms

Myosin 2, MYH2, Myosin heavy chain 2, MYHSA2, MyHC-IIa

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Validation Data Gallery

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  WB analysis of multi-cells/tissue using 55069-1-AP

  WB results of 55069-1-AP (anti-MYH2).

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  WB analysis of human skeletal muscle using 55069-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55069-1-AP (MYH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: multi-cells/tissue, human skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55069-1-AP targets MYH2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 2, skeletal muscle, adult
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_017534
• Gene Symbol: MYH2
• Gene ID (NCBI): 4620
• RRID: AB_10860267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang
pig	WB	BMC Genomics; Identification of different myofiber types in pigs muscles and construction of regulatory networks; Authors - Chenchen Li
rat	IF	Sci Rep; Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders; Authors - Shan Gao