Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20140-1-AP targets MYH4-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 4, skeletal muscle |
| Calculated Molecular Weight | 223 kDa |
| Observed Molecular Weight | 230 kDa |
| GenBank Accession Number | NM_017533 |
| Gene Symbol | MYH4 |
| Gene ID (NCBI) | 4622 |
| RRID | AB_10666434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y623 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| IHC protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| WB protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (Cat# 20140-1-AP) has accumulated 53 citations across journals including Nature Communications, Advanced Science, Science Advances, Molecular Biomedicine, International Journal of Biological Sciences, Journal of Biological Chemistry, FASEB Journal, Development, Scientific Reports, and BMC Genomics. Research fields encompass skeletal muscle biology, myogenesis, fiber-type specification and conversion, muscle atrophy/regeneration, mitochondrial metabolism, exercise physiology, nutrition, and toxicology, with studies conducted in mouse, pig, rat, human, and canine.
| Species | Application | Title |
|---|---|---|
Nat Commun Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation. | ||
Nat Commun The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization. | ||
Adv Sci (Weinh) Skeletal Muscle HSF1 Alleviates Age-Associated Sarcopenia and Mitochondrial Function Decline via SIRT3-PGC1α Axis. | ||
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Mol Biomed Inhibition of Sesn2 has negative regulatory effects on the myogenic differentiation of C2C12 myoblasts | ||
Int J Biol Sci WNT5B regulates myogenesis and fiber type conversion by affecting mRNA stability. |
