MYH4-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85376-1-RR (same clone as 85376-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85376-1-RR (MYH4-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85376-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 85376-1-RR (same clone as 85376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85376-1-RR (MYH4-Specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 85376-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 85376-1-RR (same clone as 85376-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85376-1-RR (MYH4-Specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 85376-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 85376-1-RR (same clone as 85376-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH4-Specific antibody (85376-1-RR, Clone: 242795B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85376-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 85376-1-RR (same clone as 85376-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH4-Specific antibody (85376-1-RR, Clone: 242795B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85376-1-PBS in a different storage buffer formulation.

MYH4-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 85376-1-PBS

Clone No.242795B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MYH4, Myosin 4, 242795B2, MyHC-2b, MyHC-IIb

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Product Information

85376-1-PBS targets MYH4-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 4, skeletal muscle
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: NM_017533
• Gene Symbol: MYH4
• Gene ID (NCBI): 4622
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y623
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.