MYH8-Specific Recombinant antibody

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  WB analysis of rat skeletal muscle using 84594-1-RR

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 84594-1-RR

  human heart tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 84594-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 84594-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 84594-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 84594-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYH8-Specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84594-1-RR

Clone No.241539A1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

MYH8, 241539A1, Myosin-8, Myosin heavy chain 8, Myosin 8

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, human heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84594-1-RR targets MYH8-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 8, skeletal muscle, perinatal
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 220-230 kDa
• GenBank Accession Number: NM_002472
• Gene Symbol: MYH8
• Gene ID (NCBI): 4626
• RRID: AB_3672090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13535
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.