MYH8-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis of rat skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 84594-1-RR (same clone as 84594-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

MYH8-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84594-1-PBS

Clone No.241539A1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MYH8, 241539A1, Myosin-8, Myosin heavy chain 8, Myosin 8

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Product Information

84594-1-PBS targets MYH8-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 8, skeletal muscle, perinatal
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 220-230 kDa
• GenBank Accession Number: NM_002472
• Gene Symbol: MYH8
• Gene ID (NCBI): 4626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13535
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.