MYH9 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81204-1-RR (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (81204-1-RR, Clone: 3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81204-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81204-1-RR against Human MYH9 were performed. The affinity constant is 0.297 nM.

MYH9 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 81204-1-PBS

Clone No.3E1

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

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Product Information

81204-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1612

  Product name: Recombinant human MYH9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1741-1960 aa of BC011915

  Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

• Full Name: myosin, heavy chain 9, non-muscle
• Calculated Molecular Weight: 227 kDa
• Observed Molecular Weight: 224-227 kDa
• GenBank Accession Number: BC011915
• Gene Symbol: MYH9
• Gene ID (NCBI): 4627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35579
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).