MYH9 Recombinant antibody
MYH9 Recombinant Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3E1
Cat no : 81204-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, U-87 MG cells, HeLa cells, A431 cells, U2OS cells, human placenta tissue, rat kidney tissue |
Positive IHC detected in | rat kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:4950-1:19800 |
Sample-dependent, check data in validation data gallery |
Product Information
81204-1-RR targets MYH9 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MYH9 fusion protein Ag1612 |
Full Name | myosin, heavy chain 9, non-muscle |
Calculated molecular weight | 227 kDa |
Observed molecular weight | 224-227 kDa |
GenBank accession number | BC011915 |
Gene symbol | MYH9 |
Gene ID (NCBI) | 4627 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
Protocols
Product Specific Protocols | |
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WB protocol for MYH9 antibody 81204-1-RR | Download protocol |
IHC protocol for MYH9 antibody 81204-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |