Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16287-1-AP targets MYL12A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, frog |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9413 Product name: Recombinant human MYL12A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC016372 Sequence: MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD Predict reactive species |
| Full Name | myosin, light chain 12A, regulatory, non-sarcomeric |
| Calculated Molecular Weight | 171 aa, 20 kDa |
| Observed Molecular Weight | 19-20 kDa |
| GenBank Accession Number | BC016372 |
| Gene Symbol | MYL12A |
| Gene ID (NCBI) | 10627 |
| RRID | AB_2878238 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19105 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYL12A antibody 16287-1-AP | Download protocol |
| WB protocol for MYL12A antibody 16287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYL12A Polyclonal antibody (Cat# 16287-1-AP) has 5 total citations published in EMBO J, Frontiers in Physiology, Biochemical Journal, Journal of the Association for Research in Otolaryngology, and Biofizika. The associated research fields span immunology (macrophage migration and gut-vascular barrier), smooth muscle physiology, calcium-independent muscle contraction, inner ear hair cell function, and vascular endothelial permeability. Applications cited include WB, IF, and IHC across human, mouse, pig, and frog species.
| Species | Application | Title |
|---|---|---|
EMBO J Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier | ||
Front Physiol Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction. | ||
Biochem J Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli. | ||
J Assoc Res Otolaryngol Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair Cells | ||
Biofizika [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium]. |
