MYL12A Polyclonal antibody

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  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16287-1-AP (MYL12A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16287-1-AP (MYL12A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16287-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

Epididymis secretory protein Li 24, HEL-S-24, MLC 2B, MLC-2B, MLCB

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL12A fusion protein Ag9413
• Full Name: myosin, light chain 12A, regulatory, non-sarcomeric
• Calculated Molecular Weight: 171 aa, 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC016372
• Gene Symbol: MYL12A
• Gene ID (NCBI): 10627
• RRID: AB_2878238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19105
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B).

Publications

Species	Application	Title
pig	WB	Biochem J; Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.; Authors - Shcherbakova Olga V OV
mouse	WB	Front Physiol; Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.; Authors - Jie Sun
human	IHC	Biofizika; [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].; Authors - Marchenko A V AV
mouse	WB	EMBO J; Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier; Authors - Yuanji Dai