Tested Applications
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, light chain 2, regulatory, cardiac, slow |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | NM_000432 |
| Gene Symbol | Myosin Light Chain 2 |
| Gene ID (NCBI) | 4633 |
| RRID | AB_2881342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Myosin Light Chain 2/MLC-2V Polyclonal antibody (Cat# 55462-1-AP) has 5 citations published in Cell Mol Immunol, Int J Mol Med, Stem Cell Reports (×2), and iScience. Research fields span pulmonary fibrosis, cutaneous melanoma progression, ventricular cardiomyocyte purification from embryonic stem cells, iPSC-derived cardiomyocyte maturation, and hepatic stellate cell chemotactic migration. Applications cited include WB and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis | ||
Int J Mol Med Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis. | ||
Stem Cell Reports Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. | ||
Stem Cell Reports PRDM16 modulates aspects of cell cycle dynamics and maturation in human iPSC-derived cardiomyocytes. | ||
iScience SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury. |
