Myosin Light Chain 2/MLC-2V Polyclonal antibody

Myosin Light Chain 2/MLC-2V Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55462-1-AP

Synonyms

MLC 2, MLC 2v, MLC2, MLC2v, MYL2



Tested Applications

Positive WB detected inrat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberNM_000432
Gene symbol MYL2
Gene ID (NCBI) 4633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Protocols

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.

Authors - Chao Ci
mouseIF

Stem Cell Reports

Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.

Authors - Kiwon Ban