Myosin Light Chain 2/MLC-2V Polyclonal antibody

Myosin Light Chain 2/MLC-2V Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 55462-1-AP

Synonyms

MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2/MLC-2V



Tested Applications

Positive WB detected inrat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberNM_000432
Gene symbol MYL2
Gene ID (NCBI) 4633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Protocols

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Stem Cell Reports

Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.

Authors - Kiwon Ban
humanWB

iScience

SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.

Authors - Anteneh Getachew
humanWB

Int J Mol Med

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.

Authors - Chao Ci