Validation Data Gallery
|Positive WB detected in||rat heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, light chain 2, regulatory, cardiac, slow|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||18-20 kDa|
|GenBank accession number||NM_000432|
|Gene ID (NCBI)||4633|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
|Product Specific Protocols|
|WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP||Download protocol|
|IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP||Download protocol|
|IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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