MYL3 Polyclonal antibody

MYL3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10913-1-AP


Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells
Positive FC detected inC2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10913-1-AP targets MYL3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL3 fusion protein Ag1364
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC009790
Gene Symbol MYL3
Gene ID (NCBI) 4634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)


Product Specific Protocols
WB protocol for MYL3 antibody 10913-1-APDownload protocol
IHC protocol for MYL3 antibody 10913-1-APDownload protocol
IF protocol for MYL3 antibody 10913-1-APDownload protocol
IP protocol for MYL3 antibody 10913-1-APDownload protocol
FC protocol for MYL3 antibody 10913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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