Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16052-1-AP targets MYLPF in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9013 Product name: Recombinant human MYLPF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC012571 Sequence: MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE Predict reactive species |
| Full Name | myosin light chain, phosphorylatable, fast skeletal muscle |
| Calculated Molecular Weight | 169 aa, 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC012571 |
| Gene Symbol | MYLPF |
| Gene ID (NCBI) | 29895 |
| RRID | AB_2148036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A32 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYLPF antibody 16052-1-AP | Download protocol |
| WB protocol for MYLPF antibody 16052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYLPF polyclonal antibody (Cat# 16052-1-AP) has 3 citations published in Int J Mol Sci, Mol Cell Biol, and bioRxiv. The associated research fields include ALS gene therapy and skeletal muscle function, myogenic transcription regulation by enhancer RNA, and cerebellar development involving neural lineage identity and muscle fate repression. All citations used mouse samples with WB or IF applications.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model. | ||
Mol Cell Biol MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. | ||
bioRxiv Active repression of muscle fate preserves neural lineage identity during cerebellum development. |
