MYO18A Polyclonal antibody, PBS Only

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• MYO18A Polyclonal antibody, PBS Only (14611-1-PBS)

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  WB analysis of Jurkat using 14611-1-AP (same clone as 14611-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14611-1-AP (same clone as 14611-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 14611-1-AP (same clone as 14611-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14611-1-AP (same clone as 14611-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MYO18A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14611-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA

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Product Information

14611-1-PBS targets MYO18A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6185

  Product name: Recombinant human MYO18A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1601-2002 aa of BC039612

  Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA

• Full Name: myosin XVIIIA
• Calculated Molecular Weight: 233 kDa
• Observed Molecular Weight: 230 kDa, 190 kDa
• GenBank Accession Number: BC039612
• Gene Symbol: MYO18A
• Gene ID (NCBI): 399687
• RRID: AB_2201447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92614
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.