MYO18A Monoclonal antibody

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  WB analysis using 68868-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68868-1-Ig (MYO18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MYO18A Monoclonal Antibody for WB, ELISA

Cat No. 68868-1-Ig

Clone No.1A2B12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

myosinXVIIIA, myosin XVIIIA, Molecule associated with JAK3 N-terminus, KIAA0216, CD245

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Tested Applications

• Positive WB detected in: HaCaT cells, A431 cells, SW 1990 cells, HT-29 cells, MDA-MB-468 cells, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68868-1-Ig targets MYO18A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6185

  Product name: Recombinant human MYO18A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1601-2002 aa of BC039612

  Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA

• Full Name: myosin XVIIIA
• Calculated Molecular Weight: 233 kDa
• Observed Molecular Weight: 230 kDa, 190 kDa
• GenBank Accession Number: BC039612
• Gene Symbol: MYO18A
• Gene ID (NCBI): 399687
• RRID: AB_3670442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92614
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.