MYOM3 Polyclonal antibody

MYOM3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17692-1-AP

Synonyms

Myomesin-3, Myomesin family member 3, Myomesin 3

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Validation Data Gallery

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  WB analysis using 17692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17692-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17692-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 17692-1-AP

  IP result of anti-MYOM3 (IP:17692-1-AP, 4ug; Detection:17692-1-AP 1:1000) with mouse skeletal muscle tissue lysate 1280 ug.

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  IHC staining of human skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 17692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 17692-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (17692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below

Product Information

17692-1-AP targets MYOM3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYOM3 fusion protein Ag11849
• Full Name: myomesin family, member 3
• Calculated Molecular Weight: 162 kDa, 136 kDa
• Observed Molecular Weight: 162 kDa
• GenBank Accession Number: BC067101
• Gene Symbol: MYOM3
• Gene ID (NCBI): 127294
• RRID: AB_2146624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)

Publications

Species	Application	Title
	ELISA	Nat Commun; An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species; Authors - Ai Vu Hong
mouse	WB	J Clin Invest; A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III; Authors - Antoine Gardin
mouse	WB	Nucleic Acids Res; Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides.; Authors - Karima Relizani
human	WB, IP	Hum Mol Genet; Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies.; Authors - Jérémy Rouillon
mouse	WB	Int J Mol Sci; Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy.; Authors - Nathalie Bourg
human	IF	Stem Cell Reports; A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.; Authors - Matthew J Birket