MCL1L-specific Polyclonal antibody

MCL1L-specific Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 15825-1-AP

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

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Validation Data Gallery

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  IHC staining of human spleen using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 15825-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human spleen tissue, human brain tissue, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15825-1-AP targets MCL1L-specific in IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_182763
• Gene Symbol: MCL1
• Gene ID (NCBI): 4170
• RRID: AB_2144112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. This antibody is specific to MCL1L isoform.