Mcp1 Monoclonal antibody

Mcp1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B9F7

Cat no : 66272-1-Ig

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF



Tested Applications

Positive WB detected inDC2.4 cells, LPS treated RAW 264.7 cells, mouse brain tissue, mouse lung tissue, mouse kidney tissue
Positive IHC detected inmouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66272-1-Ig targets Mcp1 in WB, IHC, IF,ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivity mouse, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mcp1 fusion protein Ag24085
Full Name chemokine (C-C motif) ligand 2
Observed molecular weight 26 kDa
GenBank accession numberNM-011333
Gene symbol Ccl2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Mcp1 antibody 66272-1-IgDownload protocol
IHC protocol for Mcp1 antibody 66272-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Theranostics

Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.

Authors - Xinzhi Li
mouseWB

Osteoarthritis Cartilage

Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB.

Authors - G Yuan
ratWB

Regen Med

Autologous nanofat transplantation accelerates foot wound healing in diabetic rats.

Authors - Li Chen
mouseWB

Biomed Pharmacother

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.

Authors - Fanchao Feng
ratWB

Mol Immunol

PMID: 31075560

Authors - Rui Liu
ratWB

Oxid Med Cell Longev

WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.

Authors - Xiaoyu Zhou