Mcp1 Monoclonal antibody

Mcp1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

75

CloneNo.

1B9F7

Cat no : 66272-1-Ig

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

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Validation Data Gallery

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  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66272-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: DC2.4 cells, LPS treated RAW 264.7 cells, mouse brain tissue, mouse lung tissue, mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 56 publications below
• IHC: See 16 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below

Product Information

66272-1-Ig targets Mcp1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mcp1 fusion protein Ag24085
• Full Name: chemokine (C-C motif) ligand 2
• Observed molecular weight: 26 kDa
• GenBank accession number: NM-011333
• Gene symbol: Ccl2
• Gene ID (NCBI): 20296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	IF	Theranostics; Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.; Authors - Xinzhi Li
mouse	WB	Osteoarthritis Cartilage; Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB.; Authors - G Yuan
rat	WB	Regen Med; Autologous nanofat transplantation accelerates foot wound healing in diabetic rats.; Authors - Li Chen
mouse	WB	Biomed Pharmacother; Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.; Authors - Fanchao Feng
rat	WB	Mol Immunol; PMID: 31075560; Authors - Rui Liu
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou