Mcp1 Monoclonal antibody
Mcp1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
mouse and More (2)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1B9F7
Cat no : 66272-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | DC2.4 cells, LPS treated RAW 264.7 cells, mouse brain tissue, mouse lung tissue, mouse kidney tissue |
Positive IHC detected in | mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 56 publications below |
IHC | See 16 publications below |
IF | See 11 publications below |
CoIP | See 1 publications below |
Product Information
66272-1-Ig targets Mcp1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse, rat, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mcp1 fusion protein Ag24085 |
Full Name | chemokine (C-C motif) ligand 2 |
Observed molecular weight | 26 kDa |
GenBank accession number | NM-011333 |
Gene symbol | Ccl2 |
Gene ID (NCBI) | 20296 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for Mcp1 antibody 66272-1-Ig | Download protocol |
IHC protocol for Mcp1 antibody 66272-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. | ||
Osteoarthritis Cartilage Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB. | ||
Regen Med Autologous nanofat transplantation accelerates foot wound healing in diabetic rats. | ||
Biomed Pharmacother Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. | ||
Oxid Med Cell Longev WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling. |