Various lysates were subjected to SDS PAGE followed by western blot with 86990-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 86990-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 86990-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 86990-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Dot Blot experiment of / using 86990-1-RR
Dot blot analysis was used to confirm the specificity of 86990-1-RR Mono/Di-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 86990-1-RR Mono/Di-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
ChIP experiment of HeLa using 86990-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono/Di-Methyl-Histone H3 (Lys9) (86990-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono/Di-Methyl-Histone H3 (Lys9) (86990-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
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PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
Protocols
Product Specific Protocols
IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 86990-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86990-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of HeLa using 86990-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 86990-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 86990-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (86990-1-RR, Clone: 252046D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
DOT BLOT Figures
Dot Blot experiment of / using 86990-1-RR
Dot blot analysis was used to confirm the specificity of 86990-1-RR Mono/Di-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
CHIP-QPCR Figures
ChIP experiment of HeLa using 86990-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono/Di-Methyl-Histone H3 (Lys9) (86990-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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