WB analysis using 86861-1-RR (same clone as 86861-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86861-1-RR (Mono-Methyl-Histone H3 (Lys23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 86861-1-RR (Mono-Methyl-Histone H3 (Lys23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 86861-1-RR (same clone as 86861-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 86861-1-RR (same clone as 86861-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Dot Blot experiment of / using 86861-1-RR (same clone as 86861-1-PBS)
Dot blot analysis was used to confirm the specificity of 86861-1-RR Mono-Methyl-Histone H3 (Lys23) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Dot blot analysis was used to confirm the specificity of 86861-1-RR Mono-Methyl-Histone H3 (Lys23) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 86861-1-RR (same clone as 86861-1-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys23) (86861-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys23) (86861-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
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WB analysis using 86861-1-RR (same clone as 86861-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86861-1-RR (Mono-Methyl-Histone H3 (Lys23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of NIH/3T3 using 86861-1-RR (same clone as 86861-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 86861-1-RR (same clone as 86861-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys23) antibody (86861-1-RR, Clone: 250368B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
DOT BLOT Figures
Dot Blot experiment of / using 86861-1-RR (same clone as 86861-1-PBS)
Dot blot analysis was used to confirm the specificity of 86861-1-RR Mono-Methyl-Histone H3 (Lys23) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
CHIP-QPCR Figures
ChIP experiment of HeLa using 86861-1-RR (same clone as 86861-1-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys23) (86861-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86861-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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